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Melanin (skin pigmentation) has also been reported to have its production reduced secondary to reduces production of the enzyme tyrosinase , which catalyzes the rate-limiting step in melanin synthesis. [44] This study noted that in B16F1 melanoma cells that incubation with up to 200ug/mL failed to exert cytotoxic effects and reduced melanin content at 25ug/mL (to 67% of control) and more-so at 50ug/mL and 100ug/mL reducing melanin down to 40% of control cells (with no significant difference between these two concentrations). [44] mRNA of tyrosinase was potently reduced to +/-% of control with 25ug/mL with higher doses being insignificantly better. [44]

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